Virus-based MicroRNA Silencing
Virus-based microRNA silencing (VbMS) is a viable and prompt method to screen and characterize the function of microRNAs (miRNAs) in plants.The Tobacco rattle virus (TRV)-based VbMS method was originally developed by the Yule Liu's WELLBETX VANILLA RA group (Sha et al., 2014) using miRNA target mimic (TM) methodology.Here, we describe the TRV-b